pLenti CMV Puro LUC (w168-1)

LOCUS       pLenti CMV Puro LUC (w168-1)                9637 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  3rd gen lentiviral Luciferase expression vector, CMV promoter, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti CMV Puro LUC (w168-1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pLenti CMV Puro LUC (w168-1)|
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     58..77
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             146..745
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(146..165)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     642..662
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             883..1116
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1188..1309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1225..1244)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1279..1298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1349..1484
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1411..1430
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(1504..1523)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1505..1523)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1533..1550)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1533..1549)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1542..1564)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1691..2146
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1778..1797)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1988..2009
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2172..2276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2277..3137
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2495..2514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3308..3896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3797..3816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4050..4067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4184..4205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4220..4250
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4258..4274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4263..4285
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4282..4298
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4282..4298
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4317..4337
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4319..4337
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4365..4591
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4592..4772
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4819..4944
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5437..5670
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6166..6282
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6305..6608
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6609..6812
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6763..6783
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6809..6833
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6917..6941
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6947..8599
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(7013..7031)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    complement(8606..8630)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8661..9249
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8714..8734)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9132..9143)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9263..125
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9298..9321)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
       61 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
      121 tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
      181 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
      241 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
      301 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
      361 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
      421 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
      481 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
      541 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
      601 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
      661 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
      721 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
      781 gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg
      841 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact
      901 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
      961 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1021 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1081 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     1141 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     1201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     1321 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1381 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     1441 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     1501 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     1561 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     1621 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     1681 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1741 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     1801 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     1861 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     1921 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     1981 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2041 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2101 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     2161 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2221 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2281 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2341 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     2401 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     2461 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     2521 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2581 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2641 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2701 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2761 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2821 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2881 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2941 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3001 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3061 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3121 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3181 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3241 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3301 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3421 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3901 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3961 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4021 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4081 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4141 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     4201 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     4261 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     4321 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     4381 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     4441 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     4501 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     4561 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     4621 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4681 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4741 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     4801 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     4861 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     4921 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     4981 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     5041 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     5101 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     5161 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     5221 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     5281 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     5341 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     5401 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     5461 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     5521 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     5581 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     5641 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     5701 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     5761 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     5821 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     5881 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     5941 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     6001 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     6061 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     6121 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
     6181 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     6241 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgataa gcttgggagt
     6301 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     6361 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     6421 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     6481 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     6541 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     6601 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     6661 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     6721 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     6781 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     6841 gacgccatcc acgctgtttt gacctccata gaagacaccg actctagtcc agtgtggtgg
     6901 aattctgcag atatcaacaa gtttgtacaa aaaagcaggc tccaccatgg aagacgccaa
     6961 aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca
     7021 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc
     7081 acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga
     7141 agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc
     7201 tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc
     7261 gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt
     7321 ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat
     7381 catccaaaaa attattatca tggattctaa aacggattac cagggatttc agtcgatgta
     7441 cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc
     7501 cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc
     7561 taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc
     7621 tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca
     7681 cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat
     7741 gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc
     7801 gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga
     7861 tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga
     7921 agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac
     7981 tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt
     8041 tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa
     8101 tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc
     8161 ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag acatagctta
     8221 ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa
     8281 aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt
     8341 cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt
     8401 tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca
     8461 agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg
     8521 tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg
     8581 cggaaagatc gccgtgtaat tctagaccca gctttcttgt acaaagtggt tgatatccag
     8641 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     8701 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     8761 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     8821 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     8881 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     8941 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     9001 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     9061 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     9121 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     9181 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     9241 cctccccgcc tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     9301 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     9361 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     9421 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     9481 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     9541 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     9601 tgggctcaga ggctgggaag gggtgggtcc gggggcg
//